![]() However, bacterial isolation is a complex and expensive technique making it difficult to be routinely used in a laboratory. Protean clinical manifestation and diagnostic challenges of human brucellosis in adults: 16 years experience in an endemic area. The samples of choice for isolation include secretions, purulent content, abomasal content of aborted fetuses, cerebrospinal fluid, pleural fluid, synovial fluid and ascitic fluid ( Mantur et al., 2006 MANTUR, B.G. Isolation of Brucella abortus is considered the most reliable diagnostic method of brucellosis. Brucellosis at the animal/ecosystem/human interface at the beginning of the 21st century. Twenty species have been described in the genus Brucella, which are classified according to their biochemical characteristics, antigenic variation and main hosts ( Godfroid et al., 2011 GODFROID, J. This disease is caused by Brucella abortus, a nonmotile gram-negative coccobacillus, with a smooth colony morphology ( Teixeira et al., 1998 TEIXEIRA, A.C.P. O WB padronizado neste estudo mostrou-se um teste sorológico com potencial uso como teste confirmatório no diagnóstico da brucelose bovina.īrucelose imunodiagnóstico proteômica sorologiaīrucellosis is an infectious disease that affects cattle and buffaloes, among other animals, causing considerable economic losses. Quando comparadas com a TFC, a sensibilidade, a especificidade e a acurácia foram 97%, 98% e 97%, respectivamente, e k= 0,929. A sensibilidade, a especificidade e a acurácia do WB, quando este foi comparado com o AAT, foram, respectivamente, 93%, 99% e 98%, e k= 0,938. O resultado da análise do WB revelou peso molecular ≤20kDa como sendo a área mais reativa e característica para identificação e separação dos animais infectados dos não infectados. Foram analisados dois grupos de amostras bovinas: grupo I, com 60 amostras de animais verdadeiros positivos e verdadeiros negativos vacinados (30 amostras positivas de animais infectados e positivos nos testes de antígeno acidificado tamponado (AAT), 2 - mercaptoetanol (2 - ME), soroaglutinação lenta em tubos (SAT) e fixação do complemento e de 30 amostras negativas no AAT) grupo II, com 383 amostras de campo, sendo 90 soropositivas e 293 soronegativas no TFC. Objetivou-se neste estudo desenvolver e padronizar um teste Western blotting (WB) para detecção de anticorpos contra B. The WB developed and standardized in the present study is a serological test with potential use as a confirmatory test for the diagnosis of bovine brucellosis.īrucellosis immunodiagnosis proteomics serologyĪ brucelose é uma doença infectocontagiosa, causada por bactérias do gênero Brucella spp., com diagnóstico baseado no emprego de técnicas sorológicas. When compared to CFT, the sensitivity, specificity and accuracy of the WB was 97%, 98% and 97%, respectively and k= 0.929. ![]() The sensitivity, specificity and accuracy of the WB compared to RBT was 93%, 99%, 98%, respectively and k= 0.938. The most reactive band in the western blotting, which properly identified and separated infected from non - infected had a molecular weight of ≤ 20kDa. Samples from two groups of cattle were analyzed: group I: 60 serum samples from true positive and true negative vaccinated animals (30 positive samples from infected animals according to rose bengal test (RBT), 2-mercaptoethanol, serum agglutination test (SAT) and complement fixation test (CFT) and 30 RBT negatives samples) group II: 383 field samples (90 positive and 293 CFT negative sera). The present study aimed to develop and standardize a western blotting (WB) test for detection of antibodies against B. with diagnosis based on use of serological techniques. Brucellosis is an infectious disease caused by bacteria of the genus Brucella spp.
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